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IDT PrimeTime Pre-designed Assays Increase qPCR Accuracy and Efficiency

Integrated DNA Technologies Inc.
26 Apr 2011
bridget bridget
Laboratory Director

Product news

Integrated DNA Technologies (IDT), the world leader in oligonucleotide synthesis, now offers PrimeTime Pre-designed qPCR Assays which are guaranteed to work for human, mouse, and rat transcriptomes. Users can search and select from the online database that contains thousands of assays.

All assays are designed to span exons, protect against non-specific amplifications, avoid known SNPs, and minimize the impact of secondary structure. Consisting of two primers and a hydrolysis probe, all three components are shipped in a single tube and are guaranteed to provide >90% efficiency.

IDT’s advanced flexibility allows users to select from five different dye/quencher combinations and choose a premixed primer to probe ratio from 1:1 to 4:1, thereby maximizing multiplexing capabilities. The design process uses the most current, clean, and complete target sequence information available to generate accurate Tm predictions and protect against off-target amplifications. Stephen Gunstream, Vice President of Marketing and Strategy, commented “We have compared our product to leading pre-designed assays and found improved efficiency, higher success rates, and increased sensitivity.”


PrimeTime Pre-designed qPCR Assays are quick and easy to order using IDT’s unique ordering tool. Each oligo undergoes 100% QC by mass spectrometry and is shipped within 2–4 working days. For more information on IDT’s PrimeTime Pre-designed qPCR Assays, please visit the company article page.

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Integrated DNA Technologies Inc.Company website

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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :

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IDT PrimeTime Pre-designed Assays Increase qPCR Accuracy and Efficiency