Identify proteins you could not identify before using new products from Promega
19 May 2009Promega introduces an innovative and simplified in-gel digestion procedure utilizing ProteaseMAX Surfactant. This new procedure offers the researcher multiple benefits:
• Save time: Digestions complete in only one hour.
• Eliminate gel extraction steps: Protein digestion and peptide recovery is complete in a single step.
• More data: Recover longer peptides which tend to remain in the gel in conventional in-gel digestion protocols.
In addition to ProteaseMAX Surfactant, Promega now offers the following new products:
• Immobilized Trypsin
• Chymotrypsin Sequencing Grade
• Lys-C Sequencing Grade
Immobilized Trypsin offers fast digestion of purified proteins or complex mixtures. Digestions are complete in as little as 30 minutes and the protocol is easily scalable to accommodate multiple protein concentrations. Chymotrypsin and Lys-C can both be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. Both proteases are suitable for digestion reactions in-solution or in-gel.
Request your free sample of Immobilized Trypsin, just visit us at ASMS booth #63 to find out how.
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ProteaseMAX™ Surfactant, Trypsin Enhancer
Promega Corp.ProteaseMax™ Surfactant is a high-quality reagent for use in protein and peptide sample preparation. ProteaseMax™ Surfactant solubilizes proteins and ensures fast and improved protein digestion with trypsin or chymotrypsin. It is designed to degrade over the course of a digestion reaction, yielding products that are compatible with downstream methods such as mass spectrometry (MS) and liquid chromatography (LC). For in-gel protein digestion, ProteaseMax™ Surfactant also improves the recovery of peptides. ProteaseMax™ Surfactant can be used with existing in-gel or in-solution digestion protocols.