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ICPL™-4-SUCCESS: The new SERVA ICPL™ Quadruplex Kit for quantitative analysis of proteomes

SERVA Electrophoresis GmbH
20 Jul 2008

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The powerful ICPL™ technology for comparative quantification of proteins is now available with a fourth label. Applying the ICPL™ quadruplex method the simultaneous quantitative analysis of four independent proteome samples can be performed by stable protein labelling side by side. The ICPL™- technology combines the power of Isotope-coded Protein Labelling (ICPL) with an unmatched dynamic range for protein identification and quantification due to its potential combination with intact protein fractionation steps. The ICPL™ quadruplex method will be introduced at HUPO 2008 in Amsterdam, The Netherlands (Halder et. al, ICPL (Isotope-coded Protein Labelling) QUADRUPLEX Technology for quantitative Proteomics). The ICPL™ Quadruplex Kit is designed for use with protein fractionation such as 1D/2D-PAGE and post digestion LC-MALDI-MS or LC-ESI-MS analysis.

To analyse ICPL labelled LC/MS data, a new software suite called ICPLQuant was developed. The main functionalities are peptide pattern searching (duplex, triplex or quadruplex), peptide quantification in an LC-run and merging with MASCOT protein results. ICPLQuant takes deisotoped data as input, generates precursor files for MS/MS and is able to import MASCOT result files for combining MS/MS and quantification results.

The SERVA ICPL Quadruplex Kit has been developed by Lottspeich et al. (MPI für Biochemie, Martinsried) and TopLab GmbH, Martinsried and is marketed worldwide through SERVA Electrophoresis GmbH and Bruker Daltonics, Bremen.

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LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.

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