Human & Mouse IgG Kits - simple, efficient antibody quantification

Guava Technologies, Inc., a leading developer of cost-effective and accessible cell-analysis systems for life science research, drug discovery, development and clinical diagnostics, has announced the introduction of RapidQuant™ IgG kits, a new line of quantitative bead-based kits designed to quantify either mouse or human IgG antibody (Immunoglobulin G) during hybridoma screening.

Guava's new RapidQuant IgG kits represent a new approach to antibody quantitation by allowing assessments to be easily performed on a plate-based benchtop flow cytometer instead of traditional plate readers. Available for both human and mouse antibodies, Guava RapidQuant IgG kits have greatly simplified protocols that require very little sample manipulation and significantly less time than traditional ELISA protocols, the method most often used for antibody quantitation. These new kits are optimised for easy use on the Guava EasyCyte™ system.

"Antibody development scientists often have to screen hundreds or thousands of hybridoma clones to select clones with the correct antibody specificity and then identify the clones producing the highest amounts of antibody. With our new RapidQuant IgG kits, scientists can easily compare IgG production on large numbers of hybridoma clones using very small volumes of supernatant." said Lawrence Lem, Ph.D., Strategic Marketing & Reagent Product Manager. "More importantly, RapidQuant IgG kits combine with other Guava assays to accelerate the entire process of antibody screening by allowing all stages of screening to be performed from the culture media in a single well, typically without having to expand clones."

The Guava RapidQuant IgG assays significantly reduce the hands-on time required to perform antibody quantitation. The simplicity of the assay and the appropriate dynamic range will greatly lower the cost of quantitation by decreasing the assay time and the labour necessary.

The Guava RapidQuant IgG kits are the first quantitative, bead-based assays for the Guava microcytometry systems. The RapidQuant IgG assay provides rapid, reproducible and accurate results in the most appropriate range for screening hybridoma culture media. Designed as universal IgG kits, the RapidQuant mouse IgG and human IgG assays quantify all IgG subtypes equivalently, saving time and money with a single assay. More information on the Guava RapidQuant Human and Mouse IgG kits is available on the article webpage.

Larry Bruder, Guava Technologies' chief executive officer, stated, "Guava's systems and assays have revolutionised cell culture monitoring and cell-based assays in the life science market. This new line of bead-based kits reflects Guava's ongoing commitment to innovate and design for ease, efficiency and cost-effectiveness."

Guava Technologies' systems, including the Guava EasyCyte and EasyCyte Mini, are affordable, highly compact, microlitre-scale cell analysis systems that offer integrated, turnkey operations. Only a few microlitres of samples, such as cultured and blood cells, are required for each experiment, saving precious cells and reagents and reducing potentially hazardous biological waste. These highly sensitive systems offer precise, accurate and easy-to-read results with often less than one day of training. The Guava EasyCyte system is ideally suited for automatic, high-throughput, 96-well microplate and multiple microcentrifuge tube analysis. Specially suited for fluorescent protein analysis, the Guava EasyCyte system allows rapid, easy screening of GFP transfectant cells. The Guava EasyCyte Mini System comes in a single-sample format and is well-suited for manual, single-sample, microcentrifuge tube analysis.

Guava Technologies, Inc., a privately held biotechnology and medical device company, is the leading provider of on-demand, easy-to-use single cell analysis systems. Guava Personal Cell Analysis (PCA™ systems, including the Guava PCA, Guava PCA-96, Guava EasyCyte and Guava EasyCyte Mini systems, are integrated, fully optimised, micro-capillary cytometry systems with embedded absolute cell counting capability. Used worldwide by the life sciences, biotechnology, and pharmaceutical industries, as well as clinical testing institutions (outside the United States and Europe), Guava's products have broad applications in scientific research and throughout the drug discovery and lead optimisation process, as well as for cell counting and optimisation of commercial bio-pharmaceutical production. Guava offers a variety of assays and dedicated software modules for the Guava PCA systems, enhancing the system's overall ease-of-use.

More information about the company and its products is available at www.guavatechnologies.com.