High Throughput Electrophoresis Unit Saves Time and Space

5 Feb 2009
Samantha Rosoman
Campaign Coordinator

Product news

A new compact electrophoresis gel system which is capable of running 4 gels in one tank to ensure the important benefits of optimum throughput, whilst saving time and space, is the latest product to be introduced by electrophoresis specialists CLEAVER SCIENTIFIC (CSL).

The CSL multiSUB4 system is designed to run up to 1200 samples in 4 vertically stacked gel trays to provide high throughput electrophoresis, while maintaining the space-saving advantages offered within the laboratory by a small unit. Each gel tray is UV-transparent to ensure easy visualisation of samples directly on the transilluminator following electrophoresis. Also, the inclusion of 4 double-sided multi-channel pipette compatible combs allows rapid sample loading from 96-well gel trays and thermal cycler blocks.

The multiSUB system is available with two formats of double-sided combs, one with one marker and one sample lane for the large scale-up of nucleic acids that may be turned over for a 12-sample throughput if required, and another included with the unit which offers both an 8 and 18 sample throughput.

The gel trays included with the CSL multiSUB are 12cm in length, while trays 6 and 18cm in length are also available for shorter and longer separations if preferred. Typical applications for the multiSUB include its use as a rapid screening accessory for DNA mini-preps prepared in 96-well plates or PCR products from thermal cycler blocks.

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ElectrophoresisElectrophoresis equipment is used to separate mixtures of protein, DNA or RNA, based on their electric charge, size and other physical characteristics, by passing them through a medium such as a polyacrylamide gel, an agarose gel or a capillary tube. Electrophoresis equipment includes horizontal and vertical gel electrophoresis chambers, isoelectric focusing systems, 2D electrophoresis and capillary electrophoresis instruments. Precast gels with a gradient can be used or gels can be hand cast. Samples are run alongside ladders or markers to identify the approximate size of a molecule. Separated proteins and nucleic acids in the gel can be stained or probed with fluorescent markers and then imaged and detected using gel documentation instruments, transilluminators, densitometers and scanners. Find the best electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gel Doc / Image AnalysisGel documentation (gel doc) or gel imaging systems are used for the analysis of proteins, antibodies and nucleic acid immobilized in polyacrylamide or agarose gels, membranes or microarrays. Explore a range of a gel imaging systems, densitometers, scanners, transilluminators or UV lamp + CCD cameras for your image analysis solutions. Colorimetric, fluorescent and/or radioisotopic samples can be visualized and documented for further analysis. See gel doc / Image analysis software for quantitative 1D and 2D analysis of your samples. Find the best gel doc / image analysis products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.