植物叶片直接进行GMO快速检测的试剂盒!

13 Jul 2007
Meng Ye
Student / Graduate

Product news

应用植物基因工程技术,在分子水平上定向重组遗传物质,改良植物性状,培育优良作物新品种,这一分子育种技术是发展现代农业的一个重要手段。在转基因植物的研究中,快速有效的检测非常重要。传统的转基因作物的检测方法是:首先提取植物基因组DNA,然后进行PCR检测。使用这一检测方法时,我们无论怎样优化基因组DNA的提取方法以及PCR条件,都无法从根本上提高转基因作物的检测速度及简化整体操作过程。
使用本试剂盒省去了植物基因组DNA的提取过程,进行PCR扩增时,直接以植物的新鲜叶片为模板,特异性地PCR扩增重组植物中的重组基因,这样可以大大缩短检测时间,减少实验过程中的交叉污染。
本试剂盒是快速检测重组作物的PCR 试剂盒,可以对转基因水稻、玉米、番茄、烟草、油菜、大豆等进行快速、简便的检测。试剂盒中的Control Template DNA可以用于Control实验,检测试剂盒的PCR扩增性能,了解PCR反应是否正常进行等。试剂盒中还含有扩增Internal Control(Actin蛋白基因)的引物,在对植物叶片进行PCR扩增时,可以从Actin蛋白基因的扩增情况来判定PCR扩增体系是否正常。

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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
植物叶片直接进行GMO快速检测的试剂盒!