ResourceLife Sciences
Four essentials of immunoassay quality
14 Sept 2026Reliable immunoassay data depends on more than sensitivity. This guide looks at four key measures of assay quality — recovery, linearity, specificity, and consistency — and how they support accurate, reproducible biomarker measurement. It also explores how R&D Systems™ by Bio-Techne assesses factors such as matrix effects, cross-reactivity, dilutional performance, and lot-to-lot reproducibility across ELISA, Luminex®, and Simple Plex™ assays.
The guide includes comparative performance data and practical examples covering recovery in complex matrices, dilutional linearity, specificity testing, and long-term assay consistency.
Resource details:
Resource type: Application note
Page count: 16
Read time: 24 mins
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ImmunologyImmunological techniques measure and characterize immune responses. Immunology kits and analysis systems often use techniques such as ELISA, radioimmunoassay (RIA) and immunodiffusion assays, Immunohistochemistry, and flow cytometry. Immunologists use equipment such as flow Cytometers, plate readers, plate washers and fluorescent microscopes.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :Biomarker DiscoveryClinical biomarkers refer to substances related to known medical conditions that can be accurately measured <i>in vitro</i>. Biomarkers can be used to diagnose presence of a disease and indicate disease severity. The discovery of new biomarkers is incredibly valuable in the field of diagnostics.ImmunoassayImmunoassays are laboratory tests that utilize antibody-antigen interactions to detect specific substances. They are widely used in diagnostics for detecting diseases, drug testing, and environmental monitoring. These assays offer rapid, sensitive, and precise detection of biomarkers, making them invaluable in clinical and research labs. Browse our peer-reviewed product directory to compare immunoassay systems, read user reviews, and get pricing directly from manufacturers.ELISAAssay DevelopmentThe process of proving an assay to be sensitive with respect to the target is known as assay development. The assay should be able to characterize novel compounds and measure the potency of these compounds against a validated biological target.

