ELISA automation with Tecan's Freedom EVOlyzer®

20 Apr 2006

Product news

The Institute for Clinical Chemistry and Hematology (IKCH) in St Gallen, Switzerland is using Tecan's new generation of ELISA automation systems, the Freedom EVOlyzer, to reduce the hands-on time of its many and varied ELISA tests.

Professor Walter Riesen, Head of IKCH, explained: "We needed an open platform that could simultaneously process multiple assays and, very importantly, it had to be able to handle short room temperature incubation times." Dr Wolfgang Korte, project leader at IKCH, elaborated: "We wanted to run a large number of different tests with only a moderate number of samples, automating as many of our applications as possible to a microtiter plate format. We needed a system that is flexible enough to handle our constantly growing and changing panel of tests."

Dr Korte concluded, "We needed an instrument that was reliable, easy to operate, provided us with clear instructions and greatly reduced hands-on time. We are confident that we have made the right choice because, once an assay is running, we can confidently leave it alone, giving us more time to spend on other tasks."

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.ELISAEnzyme-linked immunosorbent assays (ELISA), also known as enzyme immunoassays (EIA), are used for the detection and quantification of proteins, peptides and antibodies in a sample. ELISA often comes in kit format, with pre-selected antibody pairs to detect specific proteins or biomarkers of diseases such as diabetes and obesity, or cardiovascular and neurological disorders. Detection is made possible with chemiluminescent, fluorescent or colorimetric substrates and detection instruments such as microplate / ELISA readers. Other kits available include: Enzyme-linked immunospot (ELISPOT) assays for the quantification of protein-producing cells and chemiluminescent immunoassays (CLIA) kits. Find the best ELISA kits and products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
ELISA automation with Tecan's Freedom EVOlyzer®