Cut qPCR Time in Half on PCR Instruments Requiring ROX Dye

SsoFast EvaGreen Supermix with low ROX from Bio-Rad Laboratories, Inc. enhances the speed, reliability, and sensitivity of qPCR experiments performed on instruments requiring ROX reference dye. This cuts qPCR time in half on PCR instruments.

The ROX-blended version of the SsoFast EvaGreen supermix is optimized for users of PCR systems that employ ROX as a passive reference for normalization. SsoFast EvaGreen supermix with low ROX can be used effectively on Bio-Rad's real-time PCR detection systems as well as on the ABI 7500 Fast and Standard, ABI StepOne and StepOnePlus, and Stratagene MX-series real-time PCR systems.

"By pre-blending ROX into the SsoFast EvaGreen supermix, ABI 7500 users can experience the same performance advantages achieved with our SsoFast EvaGreen supermix," said Viresh Patel, Bio-Rad Senior Product Manager, PCR Reagents.

Through instant polymerase activation, optimal primer binding, and rapid polymerization kinetics, SsoFast EvaGreen supermix with low ROX can help researchers reduce their time-to-results from 90 minutes or more for standard cycling to between 35 and 45 minutes. Under these fast qPCR conditions, real-time PCR experiments maintain broad dynamic range, reliability, and accurate quantification.

The patented Sso7d-fusion polymerase utilizes the dsDNA-binding protein Sso7d to enable fast cycling without affecting PCR sensitivity, efficiency, or reproducibility. In addition, the Sso7d-fusion polymerase is significantly more resistant to PCR inhibitors, making SsoFast EvaGreen supermix with low ROX an ideal choice for applications such as qPCR directly from unpurified sample (direct PCR).