Cost-Effective, Walkaway Automation of Live Cell Assays

28 Feb 2017
Mia Harley
Biochemist

Product news

Tecan’s updated Spark® reader combines the benefits of a multimode microplate reader and a bright field imaging system in a compact package. Unlike other multimode readers, Spark lets you see what’s happening to your cells, offering automated cell imaging, confluence measurements, cell counting and viability assessments to simplify cell biology protocols and enable long-term, walkaway experiments.

Spark has been developed specifically to address the needs of cell-based workflows, and incorporates a host of features – including precise regulation of temperature, gas partial pressures and humidity – to provide an incubator-like environment and maintain optimal assay conditions. The system’s Live Viewer function turns your microplate reader into an automated microscope at the touch of a button, allowing you to instantly image and photograph defined positions in a microplate well. It offers bright field microscope-like functionality with 4x magnification and a user-definable focus, giving you another way to perform fast and easy quality control of your cells before starting your assay. In addition, Spark’s cell imaging module offers high throughput, label-free cell counting and one-click, trypan blue-based cell viability analysis using disposable Cell Chips™.

Spark also helps you to generate more reproducible cell-based assay data by allowing you to continuously monitor cell confluence within your microplate, and automatically inject a reagent once a user-defined confluence level is achieved. Together with the system’s advanced Fusion Optics – which combine the flexibility of monochromators with the sensitivity of filters – this ensures there is a Spark configuration to perfectly match your budget and workflow.

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Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Light MicroscopyLight microscopes or optical microscopes are used to visualize microscale objects under magnification, including cells, clinical specimens and materials. Lab equipment for light microscopy includes confocal microscopes, fluorescence microscopes, zoom and stereo microscopes. Microscope slides and imaging reagents are available for visualizing samples, as well as various microscope stages and incubators for large or temperature-sensitive samples. Find the best light microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Bright Field MicroscopyLive Cell ImagingLive cell imaging is the study of living cells using microscopes and high-content imaging systems. This technique provides in-depth insight into fast and complex biological processes, by allowing dynamic imaging of living cells instead of acquiring an individual image at a single point in time.Cell BiologyCell biology studies the structure, function, and behavior of cells. Understanding cellular processes is essential for research in areas such as cancer biology, stem cell research, and neurobiology. Techniques like flow cytometry, microscopy, and cell culture enable researchers to explore cellular mechanisms in detail. Browse our peer-reviewed product directory to find the best cell biology tools and equipment, compare products, check customer reviews, and get pricing directly from manufacturers.Cell Based AssaysCell-based assays use living cells to measure biological responses to various treatments or stimuli. They are essential for drug discovery, toxicology testing, and understanding cellular mechanisms. These assays are used to assess cell viability, proliferation, gene expression, and other cellular activities. Browse our peer-reviewed product directory to find the best cell-based assay tools and reagents, compare products, read customer reviews, and get pricing directly from manufacturers.