Comprehensive Expertise in HPLC

21 Feb 2012
Roger Wayman
Administrator / Office Personnel

Merck Millipore has expanded its range of information regarding high performance liquid chromatography (HPLC). The HPLC experts at Merck Millipore offer a comprehensive collection of application notes and methods, which can be found in the Analytical Application Finder on the Merck Millipore website. The company thus provides support for customers when optimizing existing processes and developing new ones.

Merck Millipore customers benefit from being able to use the HPLC products to establish reliable processes quickly with an extremely high quality standard in accordance with existing or new regulatory guidelines. The provisions of the United States Pharmacopeia (USP) are among the most significant for pharmaceutical molecules. Laboratories which apply the USP methods with HPLC columns and high purity solvents from Merck Millipore achieve reliable quality control processes quickly. Purospher STAR RP-18 HPLC columns and the LiChrosolv HPLC solvents deliver optimum results as exemplified in a recent campaign about analysis according to regulatory guidelines (Analytical HPLC for Block Buster Drugs).

LiChrosolv® Hypergrade Solvents for LC-MS

Merck KGaA, Darmstadt, Germany

High purity solvents for LC-MS and trace analysis with UV and fluorescence detection: 0.2 µm stainless steel filtered – perfect for use in UPLC and UHPLC. Very high UV-transmittance. Excellent baseline stability in gradient elution. Very low total ionic current (TIC) in LC-MS. Low level of ionic background and ion suppression. High ionization efficiency in all ionization methods (electrospray ionization/atmospheric pressure chemical ionization (ESI/APCI) positive and negative mode). Applications: Protein profiling. Pesticide Analysis. Polycyclic aromatic hydrocarbons (PAHs). Proteomics LC-MS. LC-MS routine analysis. Lichrosolv® hypergrade solvents enable analysis in the low ppb trace range and can be used for both the isocratic separation of 6 PAHs according to the German DIN method and the gradient separation of 16 PAHs according to the methods EPA 610 (analysis of drinking water) and EPA 550 + benzo(e) pyrene + perylene (analysis of waste water).

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Purospher® STAR HPLC and UHPLC columns

Merck KGaA, Darmstadt, Germany

The base silica of Purospher® STAR columns is 99.999 % pure. Furthermore, meticulous care is given to quality control over all aspects of silica structure and chemistry. The excellent converge of the surface and the purity of the material ensures excellent peak symmetry for acidic, basic and even for chelating compounds. Accurate results with excellent peak shape for all types of analytes Outstanding resolution due to high separation efficiency Proven reliability and reproducibility from run to run and batch to batch Universal compatibility with best all-round performance according to Tanaka Maximum flexibility in method development and choice of mobile phase pH stability from pH 1.5 – 10.5 Suitable for up to 100 % aqueous mobile phases Highest sensitivity and suitability for LC-MS applications

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Comprehensive Expertise in HPLC