Cisbio introduces IP-One Terbium. Discover Cisbio's new HTRF Terbium technology and assays

9 Sept 2008

Cisbio Bioassays, member of IBA group, is a global developer of technologies used in assay development and drug screening procedures. Cisbio pioneered the field of homogenous fluorescence methodologies via its proprietary technology, HTRF®, a highly-sensitive, robust technology platform that improves all research phases for new active molecules from assay development to high throughput screening and lead optimization.

Cisbio is committed to developing new functional tools for enhanced assay implementation in GPCR and kinase screening. Its second generation technology, HTRF Terbium, incorporates Lumi4™-Tb, a terbium cryptate which shows superior stability over other terbium complexes and allows for improved assay performance in terms of sensitivity, assay window, and robustness. Cisbio has already reformatted its IP-One and cAMP assays with Lumi4-Tb to create a terbium platform for assessing all GPCR targets that can be used with any existing HTRF-compatible instrument.

As part of its kinase screening portfolio, Cisbio recently introduced HTRF Transcreener® ADP, a universal and flexible assay for HTS of all kinases and ATPases, key targets in oncology research. This assay measures the generation of Adenosine 5’-diphosphate and provides enzyme-free detection for less interference and a lower false positive rate than assays which measure ATP depletion.

For more information, visit the company article page.

HTRF® Transcreener™ ADP assay

Cisbio Bioassays

ADP detection with HTRF® quality.The HTRF® Transcreener™ ADP assay is a homogeneous non-radioactive assay which measures the generation of ADP. This universal solution can be used to assess kinase and ATPase activity for High Throughput Screening and profiling applications. Enzyme-free detection allows for a low false positive rate. ADP is directly detected by a monoclonal antibody labeled with Eu3+ cryptate and correlates tightly with the amount of phosphorylated substrate for a kinase assay. HTRF® Transcreener™ ADP assay is a competitive immunoassay in which native ADP and d2 labeled ADP compete to bind to an anti-ADP MAb-cryptate.

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IP-One HTRF® Assay

Cisbio Bioassays

IP1, a downstream metabolite of IP3, accumulates in cells following Gq recepetor activation and is stable in the presence of LiCl making it an ideal GPCR (Gq) functional assay. Cisbio developed and patented a highly accurate HTRF assay for measuring IP1 in 96-, 384- and 1536-well formats providing for a HTS compatible assay.

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IP-One Terbium kits

Cisbio Bioassays

In order to provide you with the most advanced tools for GPCR cell-based assays HTRF Technology now incorporates Lumi4®-Tb chemistry. Lumi4®-Tb is the first and only TR-FRET terbium cryptate, and is fully compatible with all existing HTRF readers. Therefore IP-One Tb offers new levels of sensitivity and performance in cell-based assays.

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