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Cell-free nucleic acid extraction

AMSBIO
22 Jan 2021
Ellen Simms
Product and Reviews Editor

Product news

The cfPure® Cell Free DNA Extraction Kit from AMSBIO is a magnetic bead-based DNA extraction kit designed to isolate circulating cell-free DNA (cfDNA) from human plasma or serum samples.

Using a quick, simple, and automation friendly protocol the kit allows users to quickly extract high quality cfDNA that is ready for use in downstream applications including next-generation sequencing, Bisulfite Sequencing, PCR, qPCR, ddPCR and other demanding applications. This optimized protocol gives highly reproducible results and higher yields relative to other magnetic bead-based methods.

Using silica-coated paramagnetic particles - cfPure® kits were developed to ensure efficient recovery of 100bp-500bp DNA fragments in order to maximize recovery of low molecular weight cell-free DNA. Designed for scalability (100 µl to 10 mL of serum or plasma), cfPure® kits enable you to use just the number of beads and amount of reagent that you need for your cfDNA extraction.

As well as being simple to use, scalable and easy to automate, cfPure® kits allow you to use less magnetic bead solution if you are purifying small amounts of plasma – saving you money. Since cfDNA is typically found at very low concentrations and in a fragmented state, very efficient DNA capture, as provided by cfPure® Cell Free DNA Extraction Kits, is vital to provide sensitive and reliable results.

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DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.qPCRQuantitative PCR (qPCR) is a molecular biology technique used to amplify and measure the amount of a specific DNA or RNA target. It plays a crucial role in gene expression analysis, pathogen detection, and quantifying DNA or RNA in various applications. Real-time PCR provides precise, quantitative results and is widely used in research, diagnostics, and clinical studies. Browse our peer-reviewed product directory to find the best qPCR solutions; compare products, check reviews, and get pricing directly from manufacturers.DNA ExtractionDNA extraction is a critical step in many biological studies, including genomics, diagnostics, and forensic analysis. The process involves isolating DNA from cells or tissues, and the quality and purity of the extracted DNA can impact downstream applications. Explore DNA extraction products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

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