Catch the “Buzz” with the New <i>Trans</i>IT®-Insect Transfection Reagent from Mirus Bio

16 Dec 2013
Sarah Thomas
Associate Editor

If low protein expression in insect cells is bugging you, consider the NEW TransIT®-Insect Transfection Reagent. Insect cells are a powerful platform for generating recombinant baculovirus and for producing high-levels of soluble, functional protein. TransIT-Insect is an animal-origin free transfection reagent specifically optimized for high gene expression in a variety of insect cell types with distinct advantages over other reagents such as:

Exceptional DNA Delivery - In insect cell types including Sf9, High Five™ and S2
High Baculovirus Production - Ideal for baculovirus expression in insect cells
Serum Compatibility - Non-liposomal, animal-origin free formulation that eliminates media change
Better Value - Low reagent amounts required per transfection

“Many of the reagents addressing insect cell transfection are cationic-lipid formulations and yield low efficiency in serum containing media,” said Scott Hayes, Vice President, Mirus Bio LLC. “As our customers depend on the non-liposomal nature of TransIT® transfection technologies, they can be assured that TransIT-Insect will meet their expectations for high efficiency and robust protein production.”

In head-to-head testing, transfections with TransIT-Insect show at least 2-fold better protein expression in insect cells.

For experimental details and additional testing results, please visit www.mirusbio.com

TransIT®-Insect Transfection Reagent

Mirus Bio LLC

High yield transient transfection and baculovirus titers in insect cells. Insect cell expression is a platform used to produce proteins with simple post-translational modifications. Transient transfection and recombinant baculovirus production are commonly used methods for insect cell expression.TransIT®-Insect Transfection Reagent is an animal-origin free transfection reagent specifically optimized for high gene expression in a variety of insect cell types.TransIT®-Insect Transfection Reagent Features: Exceptional DNA Delivery - In insect cell types including Sf9, High Five™ and S2 High Baculovirus Production - Ideal for baculovirus expression in insect cells Serum compatibility - Non-liposomal, animal-origin free formulation that eliminates media change Better Value - Low reagent amounts required per transfection

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