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Brilliant Gel Visualization with Visi-Blue Technology

Analytik Jena GmbH+Co. KG
18 Jul 2011
bridget bridget
Laboratory Director

Product news

UVP, LLC announces enhanced visualization and imaging capabilities with new Visi-Blue™ technology. Visi-Blue transillumination is recommended for viewing a wide range of fluorescent stained gels which emit light at 460-470nm, including GelGreen™, SYBR® Green, SYBR Gold, SYBR Safe, GelStar®, SYPRO® Ruby, SYPRO Orange and other fluorescein stains.

Visi-Blue technology, including Transilluminators and Converter Plates, does not produce UV light and therefore eliminates UV exposure and minimizes damage to samples. Furthermore, Visi-Blue technology is UV-safe, allowing researchers to perform experiments without concern about harmful UV effects.

The Visi-Blue instruments' specially designed filter glass and lighting combination enable bright gel illumination and excitation. Visi-Blue light reduces background noise and increases image signal, creating a higher image contrast for enhanced visualization and analysis. An amber cover, included with Visi-Blue Transilluminators and optional on Converter Plates, absorbs the blue excitation and provides a clear view of the stained gel's brilliant fluorescence. When viewing the fluorescent gel through the amber cover, researchers can easily see the critical aspects of the sample, allowing for optimal band cutting. For researchers that currently use a UV transilluminator, the Visi-Blue Converter Plate converts UV to a safe 460-470nm excitation wavelength. The Visi-Blue instruments are available in a variety of filter sizes to accommodate gels ranging from 21x26cm to 20x40cm.

For researchers photographing gels using Visi-Blue instruments with a UVP gel imaging system, a UVP-proprietary and unique camera filter is provided with all Visi-Blue equipment. Captured gels clearly display the sensitivity and high image contrast that Visi-Blue instruments provide.
According to Mike Capps, UVP’s Marketing Product Manager, “Visi-Blue technology allows lab researchers to use a wide variety of newer and safer stains for fluorescence imaging. These stains, described above, are much safer than Ethidium Bromide, which is both a known carcinogen and mutagen. By eliminating EtBr from the lab setting, user safety is increased, hazardous material control costs are significantly decreased, and both mutagenic and UV damage to samples are virtually eliminated. Many of today’s advanced laboratories have begun using this new fluorescence technology, and UVP is proud to offer an innovative and flexible array of solutions to meet these demands.”

UVP's Visi-Blue technology supplies a UV safe method for visualization and capture of a wide range of fluorescent stains. For further information on UVP's Visi-Blue instruments, contact UVP, LLC via the company article page.

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Analytik Jena GmbH+Co. KGCompany website

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ElectrophoresisElectrophoresis equipment is used to separate mixtures of protein, DNA or RNA, based on their electric charge, size and other physical characteristics, by passing them through a medium such as a polyacrylamide gel, an agarose gel or a capillary tube. Electrophoresis equipment includes horizontal and vertical gel electrophoresis chambers, isoelectric focusing systems, 2D electrophoresis and capillary electrophoresis instruments. Precast gels with a gradient can be used or gels can be hand cast. Samples are run alongside ladders or markers to identify the approximate size of a molecule. Separated proteins and nucleic acids in the gel can be stained or probed with fluorescent markers and then imaged and detected using gel documentation instruments, transilluminators, densitometers and scanners. Find the best electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.ELISAEnzyme-linked immunosorbent assays (ELISA), also known as enzyme immunoassays (EIA), are used for the detection and quantification of proteins, peptides and antibodies in a sample. ELISA often comes in kit format, with pre-selected antibody pairs to detect specific proteins or biomarkers of diseases such as diabetes and obesity, or cardiovascular and neurological disorders. Detection is made possible with chemiluminescent, fluorescent or colorimetric substrates and detection instruments such as microplate / ELISA readers. Other kits available include: Enzyme-linked immunospot (ELISPOT) assays for the quantification of protein-producing cells and chemiluminescent immunoassays (CLIA) kits. Find the best ELISA kits and products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Gel Doc / Image AnalysisGel documentation (gel doc) or gel imaging systems are used for the analysis of proteins, antibodies and nucleic acid immobilized in polyacrylamide or agarose gels, membranes or microarrays. Explore a range of a gel imaging systems, densitometers, scanners, transilluminators or UV lamp + CCD cameras for your image analysis solutions. Colorimetric, fluorescent and/or radioisotopic samples can be visualized and documented for further analysis. See gel doc / Image analysis software for quantitative 1D and 2D analysis of your samples. Find the best gel doc / image analysis products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :

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Brilliant Gel Visualization with Visi-Blue Technology