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Bioo Scientific Launches ELISAs for High-Throughput ApoB Quantitation

25 Apr 2010
Sarah Sarah
Marketing / Sales

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Bioo Scientific introduces a new MaxDiscovery™ Apolipoprotein B (ApoB) ELISA Kit, a complete, two-step ELISA that analyzes the quantity of ApoB in tissues, serum, plasma or cultured cells for research purposes. ApoB is the primary apolipoprotein of LDL, low-density lipoproteins or bad cholesterol, which is responsible for carrying cholesterol to tissues. This kit offers precise protein measurement along with the ability to analyze multiple samples in low-volume, high-throughput experiments.

According to Dr. Masoud Toloue, Senior Scientist at Bioo Scientific, “Elevated plasma concentration of Apolipoprotein B (ApoB) is an established risk factor for atherosclerosis and a better indicator of heart disease than total cholesterol or LDL alone making it a highly sought after pharmaceutical lipid target. Considerable evidence also suggests that ApoB should be measured after statin therapy, as low HDL cholesterol and otherwise normal plasma lipids can coexist with an elevated level of ApoB.”

The MaxDiscovery ApoB ELISA Kit It uses a two-step sandwich ELISA format which increases reproducibility and reduces errors. It is available in 96-well and 384-well formats; both of which have plates that are pre-coated with capture monoclonal antibodies and contain all of the reagents needed to perform the assays. For increased flexibility, the 96-well plate is composed of 8-well breakaway strips.

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ELISAEnzyme-linked immunosorbent assays (ELISA), also known as enzyme immunoassays (EIA), are used for the detection and quantification of proteins, peptides and antibodies in a sample. ELISA often comes in kit format, with pre-selected antibody pairs to detect specific proteins or biomarkers of diseases such as diabetes and obesity, or cardiovascular and neurological disorders. Detection is made possible with chemiluminescent, fluorescent or colorimetric substrates and detection instruments such as microplate / ELISA readers. Other kits available include: Enzyme-linked immunospot (ELISPOT) assays for the quantification of protein-producing cells and chemiluminescent immunoassays (CLIA) kits. Find the best ELISA kits and products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.High-Throughput ScreeningHigh-throughput screening (HTS) is an automated drug discovery technique for identification of active compounds against a compound library. Use HTS readers and integrated assay preparation / analysis workstations to screen your compounds. Identify active compounds against various HTS libraries, including membranes, proteins and peptides and HTS cell lines. Find the best high-throughput screening products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.High-Content ScreeningHigh-content screening (HCS), also known as high-content analysis (HCA), is a high-throughput technique used in drug discovery to identify substances that alter the phenotype of cells. HCS uses fluorescent microscopic imaging and automated image analysis to investigate cellular events such as apoptosis, cell viability, GPCR activation, oxide production, neurite outgrowth, and cell signaling. Find the best fluorescent labeling reagents, cellular assays, and high-content imaging systems in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Point-of-CarePoint-of-Care Testing (POCT) or Near Patient Testing (NPT) products are available for urine, blood and other clinical chemistry analyses. POCT includes: blood glucose testing, blood gas and electrolytes analysis, rapid coagulation testing (PT / INR), rapid cardiac markers diagnostics, drugs of abuse screening, urine strips testing, pregnancy testing, fecal occult blood analysis, food pathogens screening, hemoglobin diagnostics, infectious disease testing and cholesterol screening.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Clinical TrialsClinical Trials, an essential part of drug discovery process, assess the safety and effectiveness of a new medication or device in the pharmaceutical industry. Clinical Trials are a phased process (Phase 0, Phase I, Phase II, Phase III and Phase IV) which begins after initial preclinical testing.

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Bioo Scientific Launches ELISAs for High-Throughput ApoB Quantitation