Bioline Launches the MyTaq™ Extract-PCR Kit, Single Tube Extraction of PCR-Ready DNA from Mammalian Tissues
4 Jul 2013Bioline, The PCR Company, a wholly-owned subsidiary of Meridian Bioscience, Inc., is proud to announce the worldwide release of the MyTaq™ Extract-PCR Kit.
Powered by the high performance MyTaq™ DNA Polymerase, the MyTaq™ Extract-PCR Kit offers a quick and easy alternative for the extraction and amplification of DNA from a variety of mammalian tissue types. Researchers using the MyTaq™ Extract-PCR Kit can replace complicated and time consuming extraction methods with a simple 15 minute, single tube, protocol; making it perfect for high throughput applications, such as mouse genotyping and sequencing.
Marco Calzavara, President of Bioline commented, "With the MyTaq™ Extract-PCR Kit; researchers can now quickly achieve the purity and yield necessary for high-performance PCR and real time PCR experiments from solid tissues, such as mouse tail or mouse ear. This makes MyTaq™ extract the perfect addition to our widely used PCR reagents.”
Richard L. Eberly, President of Meridian Life Science, Inc., stated, “We are delighted to release the MyTaq™ Extract-PCR Kit, another product in the rapidly expanding portfolio of highly specialized molecular biology reagents from Bioline. We are committed to our life science customers and to bringing innovation and quality products; such as the family of MyTaq™ products to the research laboratory, clinical diagnostic laboratories, and biotechnology companies.”
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MyTaq™ Extract-PCR Kit
Meridian Bioscience Inc.Many DNA extraction methods can be laborious and time consuming, involving phenol extraction, overnight incubations often combined with column purification. MyTaq Extract-PCR Kit offers a rapid and easy alternative for the extraction and amplification of DNA from a variety of tissue types, particularly solid tissues such as mouse tail or mouse ear. The DNA extractions are performed in a single-tube, without the need for multiple washing steps, greatly reducing the risk of sample loss and contamination. The extracted DNA is amplified using MyTaq HS Red Mix, the latest generation of very high performance polymerase mastermixes unique to Bioline, powered by antibody mediated hot-start to reduce non-specific amplification. The advanced formulation of MyTaq HS Red Mix allows fast cycling conditions to be used, greatly reducing the reaction time without compromising PCR specificity or yield.Together, the single tube lysis protocol and MyTaq HS Red Mix maximizes sensitivity, while minimizing contamination risk, significantly reduces reaction times as well as delivering improved success rates in protocols such as mouse DNA characterization. The specially designed MyTaq HS Red formulation does not interfere with the PCR reaction but allows users to load samples directly onto a gel after the PCR without the need to add loading buffer. The red dye migrates like a 350bp fragment on a 2% agarose TAE gel (or 600bp on a 1% agarose). Features and Benefits • Rapid extraction protocol - High-yield PCR-ready DNA in 15 minutes • Replaces complicated DNA extraction procedures • Convenient single-tube extraction minimizes contamination • Coupled with MyTaq™ HS Red Mix for fast and highly-specific amplification Applications • Ideal for high-throughput genotyping from mammalian tissues • Detection of transgenes • Knockout analysis