Bio-Rad iQ-Check Aspergillus Real-Time PCR Detection Kit receives AOAC International approval

The kit detects four hazardous Aspergillus species in cannabis flower

25 May 2021
Diane Li
Assistant Editor

Product news

Bio-Rad Laboratories, Inc. has announced that AOAC INTERNATIONAL has approved Bio-Rad’s iQ-Check Aspergillus Real-Time PCR Detection Kit for the detection of four hazardous Aspergillus species: A. flavus, A. fumigatus, A. niger, and A. terreus in cannabis flower and in both nonsolvent- and solvent-based cannabis concentrates.

The iQ-Check Aspergillus Real-Time PCR Detection Kit is validated by the AOAC Research Institute under its Performance Tested Method program. To obtain the validation, a study was performed that resulted in no significant difference between the iQ-Check Aspergillus method and the reference method. The kit was evaluated on parameters such as robustness, product consistency, stability, inclusivity and exclusivity, and matrix studies. In addition, Bio-Rad’s iQ-Check Free DNA Removal Solution was validated as part of the workflow for testing cannabis flower.

The fast and accurate test uses a simple workflow that includes gene amplification and real-time PCR detection. After enrichment and DNA extraction, the test harnesses the sensitivity and specificity of Bio-Rad’s PCR technology to perform real-time PCR. Next, Bio-Rad’s CFX Manager IDE Software automatically generates and analyzes the results.

Three other kits in Bio-Rad’s cannabis microbial testing product portfolio recently received AOAC approval for cannabis flower matrix: the iQ-Check Salmonella II, iQ-Check STEC VirX, and iQ-Check STEC SerO II PCR Detection Kits.

Please visit bio-rad.com/cannabis to learn more about the iQ-Check Aspergillus Real-Time Detection Kit and Bio-Rad’s complete range of iQ-Check real-time PCR test kits for cannabis microbial testing.

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iQ-Check Salmonella II PCR Detection Kits

Bio-Rad

The iQ-Check Salmonella II PCR Detection Kit is based on the fast, sensitive, and proven technology of real-time PCR. The kit is intended for detecting Salmonella spp. in animal feed and environmental samples, as well as in food samples such as poultry, eggs, and raw meats. Due to the sensitivity and specificity of PCR, results can be obtained in little as 12 hr for raw meat samples and in 20 hr for all other samples following a single enrichment in a nonselective medium. Highly Specific and Reliable The iQ-Check Salmonella II Kit uses an optimized system of primers and probes to ensure high specificity and eliminate cross-reactions. It is designed as a multiplex reaction that includes an internal inhibition control that is amplified in parallel with the target DNA for a reliable result. Maximum Flexibility Developed as an open and flexible system, the iQ-Check Salmonella II Kit can be used for up to 94 samples. Reactions can be run on low- or high-throughput Bio-Rad instruments, depending on the laboratory’s needs. Different iQ-Check assays can be run in parallel on the same plate. Validated Protocols The iQ-Check Salmonella II protocol has been validated by several certification bodies. These validation studies have shown that the kit is equivalent to or better than the various reference methods evaluated and provides results in much less time. A Complete Solution Bio-Rad offers a complete solution with standardized reagents, such as enrichment media, PCR kits, confirmation agars, and PCR detection systems, in addition to software for automated analysis specific to each test. Kit Components Lysis reagent for DNA extraction Fluorescent probes PCR amplification mix Positive and negative PCR controls

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Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Toxicology / Drugs of Abuse TestingToxicology and Drugs of Abuse Testing is the investigation into toxic and illegal substances found at the scene of a crime or from a suspect and / or victim associated with a scene of a crime. Analyzers, immunoassays and drugs of abuse test kits and presumptive test kits are an essential aspect of toxicology investigation.RegulatoryPharmaceutical regulations impact on all areas of drug development, manufacture and control and supply. Services are provided from the early stages of drug development and clinical trials through to dossier submission, approval and marketing. Regulatory also includes submission of analytical studies from the pharmaceutical product and safety. PCRMicrobiologyCannabinoidsCannabinoids are compounds found in cannabis that have therapeutic effects, such as pain relief, anti-inflammatory properties, and potential neuroprotective benefits. Research into cannabinoids is growing, particularly in the fields of pain management, neurological diseases, and cancer treatments. Browse our peer-reviewed product directory to find the best cannabinoid research tools, compare products, check reviews, and get pricing directly from manufacturers.Real-Time PCRReal-time PCR (RT-PCR) is a technique used to amplify and simultaneously quantify nucleic acids in real time. It is a key tool in gene expression analysis, pathogen detection, and diagnostics. RT-PCR allows for the detection of specific RNA or DNA sequences with high sensitivity and specificity. Browse our peer-reviewed product directory to find the best Real-Time PCR systems; compare products, check reviews, and get pricing directly from manufacturers.Cannabis