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Bio-Rad Announces Availability of a Western Blotting Standard and Chemiluminescent Detection Kit for Protein Size and Expression Analysis

Bio-Rad
4 Apr 2007

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Bio-Rad Laboratories, Inc., a multinational manufacturer and distributor of life science research products and clinical diagnostics, today announced the release of a broad-range, prestained chemiluminescent protein standard and a chemiluminescent detection kit for protein sizing and expression analysis.

The Precision Plus Protein™ WesternC™ standards offers ten prestained protein bands that can also be detected through chemiluminescent western blotting. The Immun-Star WesternC kit is a complementary chemiluminescent detection kit that is optimized for CCD imaging of the WesternC standards and protein samples. Currently, Bio-Rad offers a sample size of both the WesternC standard and the Immun-Star WesternC kit.

Bio-Rad envisions that the Precision Plus Protein WesternC standard will become the leading western blotting standard in all areas of biological research. The complementing Immun-Star™ WesternC™ kit will further establish Bio-Rad as a leading provider of protein standards and allow users to visualize the standards on CCD imagers.

The Precision Plus Protein WesternC Standard
Applicable for all 1-D gel electrophoresis experiments, the Precision Plus Protein WesternC Standard combines the convenience of prestained bands with western blot detection. This allows users to monitor electrophoresis progress, assess transfer efficiency and direct visualization of the WesternC standards through Western blotting.

The Precision Plus Protein WesternC standard offers ten prestained protein bands over the same broad range as all Precision Plus Protein standards, i.e. from 10 to 250 kD. The 25, 50 and 75 kD bands are stained pink for easy band reference and blot orientation, with all other bands prestained blue. Each standard has an integral Strep-tag sequence to enable parallel detection on western blots using StrepTactin-HRP conjugates, also provided by Bio-Rad.

Optimized for CCD imagers, the Precision Plus Protein WesternC standards are the sharpest in the industry and ideal for visualization of blots, production of publication-quality images of bands on blots, and accurate molecular weight estimation direction from acquired blot images.

To utilize and complement the Precision Plus Protein WesternC standards, the Immun-Star WesternC kit is specifically designed for CCD imagers. The Immun-Star WesternC kit generates an intense signal immediately that is easily captured and repeatedly imaged by CCD cameras such as Bio-Rad’s Molecular Imager® VersaDoc™ and ChemiDoc™ XRS Systems. The Immun-Star WesternC kit allows researchers to take full advantage of the features offered by their imaging instruments.

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ElectrophoresisElectrophoresis equipment is used to separate mixtures of protein, DNA or RNA, based on their electric charge, size and other physical characteristics, by passing them through a medium such as a polyacrylamide gel, an agarose gel or a capillary tube. Electrophoresis equipment includes horizontal and vertical gel electrophoresis chambers, isoelectric focusing systems, 2D electrophoresis and capillary electrophoresis instruments. Precast gels with a gradient can be used or gels can be hand cast. Samples are run alongside ladders or markers to identify the approximate size of a molecule. Separated proteins and nucleic acids in the gel can be stained or probed with fluorescent markers and then imaged and detected using gel documentation instruments, transilluminators, densitometers and scanners. Find the best electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Gel Doc / Image AnalysisGel documentation (gel doc) or gel imaging systems are used for the analysis of proteins, antibodies and nucleic acid immobilized in polyacrylamide or agarose gels, membranes or microarrays. Explore a range of a gel imaging systems, densitometers, scanners, transilluminators or UV lamp + CCD cameras for your image analysis solutions. Colorimetric, fluorescent and/or radioisotopic samples can be visualized and documented for further analysis. See gel doc / Image analysis software for quantitative 1D and 2D analysis of your samples. Find the best gel doc / image analysis products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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