Axygen Introduces Novel Screening Reagent
27 May 2009Axygen introduces the first ever spray bottle for application of XGal-IPTG mixture that will take the pain out of plate preparation. This proprietary mixture not only ensures the stability of XGal and IPTG but gives clean round colonies for easy screening. No more measuring, mixing or using loops for application; just spray 3 times on the agar plate. Each spray delivers precise and optimal volumes of XGal-IPTG mixture maintaining consistency between plates. Each spray bottle has 20ml of XGal-IPTG mixture sufficient for 100 standard size plates. All the for about the same cost as most X-Gal packs.
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X-Gal-IPTG Spray
Axygen Scientific, Inc.Axygen X-Gal IPTG spray is a convenient ready-to-use solution of optimal quantities of IPTG, X-Gal and stabilizers presented in a spray bottle for easy, accurate and consistent screening of Blue/White colonies. Advantages of Axygen X-Gal-IPTG Spray include:• Easy, accurate and convenient. • Sprays deliver optimal concentration and volume consistently for consistent and accurate results. • No need to weigh X-Gal and IPTG and mixing them in special solutions saving time and cost. • Proprietary mixtures of stabilizers ensure the mixture is stable for 6 months when stored under recommended conditions. Applications of Axygen X-Gal-IPTG Spray:Colony screening of blue and white colonies in expression studies. Axygen X-Gal-IPTG Spray Storage: 4 °C. Shipped at ambient. Stability of Axygen X-Gal-IPTG Spray: 6 months when stored at 4 °C. Protocol for Axygen X-Gal-IPTG Spray X-Gal-IPTG spray is a ready to use solution that can be directly sprayed on agar plates containing appropriate antibiotics. Spray solution in provided a brown bottle and it is enough for approximately 100 plates (100 mm size). Keep a distance of about few inches (between spray bottle and plates) while spraying solution on agar plates. Spray the solution 3-4 times (~200 μl) on each agar plate, which is equivalent to about 1.3 mg of each of X-Gal and IPTG per plate.