Amsbio introduces StemFit Basic 05 cell culture medium

Amsbio has launched StemFit™ Basic05, a next-generation, animal origin-free medium designed for scalable human pluripotent stem cell (hPSC) manufacturing.

Compatible with both 2D adherent and 3D suspension culture, this cell culture medium enables a streamlined workflow from cell expansion to process scale-up using a single medium. The use of a single medium across culture modes minimizes the need for process re-development and optimization traditionally associated with media changes.

Beneficially, pluripotent stem cells cultured in StemFit™ Basic05 also retain their differentiation capability and have been confirmed to be capable of differentiation directly from 3D aggregates.

For labs looking to implement stable and scalable suspension culture and scalable processes – high cell viability and uniform spheroid formation are vital prerequisites. StemFit™ Basic05 has been demonstrated to reliably deliver high cell viability and uniform spheroid formation across multiple human iPSC lines, along with maintenance of a normal karyotype.

Offering robust and highly reproducible uniform spheroid formation – StemFit™ Basic05 also reduces process complexity and development burden. It supports flexible culture scheduling, with comparable cell expansion even when weekend feeding was omitted.

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