Abcam’s New Alexa Fluor Secondary Antibodies Provide Increased Selection for Multi-Color Staining

4 Nov 2013
Sarah Thomas
Associate Editor

Product news

Abcam Plc., a leading provider of protein research tools and services, announced today that it has added new Alexa Fluor® 405/568/750 conjugated secondary antibodies to its existing Alexa Fluor® 488/555/594/647 conjugates. Extensively tested in Abcam’s labs, the Alexa Fluor range guarantees bright staining and low background. The different color variants were selected to provide an increased selection for multi-color staining.

The Alexa Fluor portfolio comprises antibodies raised against mouse/rabbit/rat/chicken/goat/sheep/guinea-pig IgG and against mouse/rabbit IgM. It also includes a large selection of pre-adsorbed/cross-adsorbed and F(ab’)2 fragment secondaries to ensure low species cross-reactivity. A dilution range of 1/200 -1/1000 in IF/ICC allows for at least 250 stainings* per product to improve cost efficiency.

Judith Langenick, Product Manager, Secondary Antibodies, commented: “The Alexa range expansion adds a blue emitting fluorophore, Alexa Fluor® 405, and a near IR dye, Alexa Fluor® 750. The addition of Alexa Fluor® 568 is ideal for confocal laser-scanning microscopists, as most of these microscopes have a 561nm laser, which optimally excites the Alexa Fluor® 568 dye. We’re pleased that we can offer our customers more dye variants and enable them to perform multi-color imaging experiments.”

Abcam Alexa Fluor secondaries are ideal for cell biologists and microscopists undertaking multi-color staining from all research backgrounds. The addition of these products complements Abcam’s innovative RabMAb® (rabbit monoclonal antibodies) products and market leading primary antibody range.

Abcam Alexa Fluor secondaries are accompanied with information-rich datasheets, fast delivery, and expert scientific support. They are available globally and can be purchased directly through the Abcam website or through one of Abcam’s authorized distributors.

*assuming a 1/200 dilution in 200 µl PBS

Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Labware and Lab SuppliesLabware and lab supplies include various forms of glassware, plasticware, reagents and personal protective equipment (PPE) and are used across all scientific laboratories, enabling daily tasks to be performed. Explore a range of flasks and beakers, petri dishes, carboys and storage bottles. Labware such as condensers, desiccators and burettes are also available for more specific tasks. Find the best labware and lab supplies in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Light MicroscopyLight microscopes or optical microscopes are used to visualize microscale objects under magnification, including cells, clinical specimens and materials. Lab equipment for light microscopy includes confocal microscopes, fluorescence microscopes, zoom and stereo microscopes. Microscope slides and imaging reagents are available for visualizing samples, as well as various microscope stages and incubators for large or temperature-sensitive samples. Find the best light microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ImmunohistochemistryImmunohistochemistry (IHC) is a technique used to detect specific proteins in tissue samples by using antibodies that bind to target antigens. IHC is widely applied in pathology, immunology, hematology and cancer diagnostics. Explore IHC tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.ConfocalSecondary Antibodies
Abcam’s New Alexa Fluor Secondary Antibodies Provide Increased Selection for Multi-Color Staining