The Impact of Transfection Mediated Toxicity - Gene Expression and Cytotoxicity Analysis of Transfection Reagents
7 Feb 2013While plasmid DNA delivery is a widely used method to study cellular functions of proteins of interest, studies to identify nontoxic gene delivery reagents are limited. This application note presents a study which explores the conventional toxicological endpoints, and identifies the minimal-transcriptomic effects, of TransIT®‐LT1, TransIT®‐2020 and Lipofectamine® 2000 Transfection Reagents using quantitative reverse transcriptase PCR (RT‐qPCR) array and pathway analysis software.
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TransIT®-2020 Transfection Reagent
Mirus Bio LLCA high performance, animal-free, broad spectrum DNA transfection reagent. Broad Spectrum DNA Delivery - Achieve high expression in many cell types, including hard to transfect cell lines and primary cells Outperforms Competitor Reagents - TransIT®-2020 demonstrated higher protein yield when compared to FuGENE® HD, Lipofectamine™ 2000, and Lipofectamine™ 2000 CD Superior Transfection of Insect Cells - Obtain higher expression than other insect cell transfection reagents Animal Origin Free - TransIT®-2020 provides high performance with maximum compatibility
TransIT®-LT1 Transfection Reagent
Mirus Bio LLCTransIT®-LT1 Transfection Reagent from Mirus Bio- A broad spectrum, low toxicity, DNA transfection reagent • Broad Spectrum DNA Delivery - Utilize one transfection reagent and protocol for a variety of cells. • Low Cellular Toxicity - Maintain cell density and reduce experimental biases. • High Efficiency Delivery - Achieve expression in a large population of cells for experimental success. • Deliver Single or Multiple Plasmids - Suitable for many applications such as gene expression, shRNA expression, viral production, and promoter analysis.