The Benefits Gained from Using a High Throughput Microseeding Technique for Protein Crystallization

Protein crystallisation is a major bottleneck for protein structure determination, and it still remains the least standardised part of the protein structure determination process by X-ray crystallography. Nucleation is the first and critical step to succeed with protein crystallisation. Crystal nuclei (seeds) can be transferred from drop to drop to increase success with the 3D growth of crystals. This seeding technique was mentioned in 1976 by Blundell and Johnson, where the standard microseeding technique was performed with a 10-fold dilution of crushed crystals.