Selecting Buffers to Remove Uncertainty in Tryptic Digestion
Selecting Buffers to Remove Uncertainty in Tryptic Digestion
4 Nov 2015Protein digestion is a fundamental technique employed in biopharmaceutical and proteomic applications. It is used to analyze the sample and identify structural features or post-translational modifications (PTMs). Despite its widespread use, protein digestion still provides many analytical challenges. This application note demonstrates how Thermo Scientific™ SMART Digest™ kits remove uncertainty associated with conventional solution-based tryptic digestion protocols, resulting in higher reproducibility and sample characterization.
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Thermo Scientific™ SMART Digest™ Kits
Thermo Fisher ScientificObtain high quality analytical results from protein digests by using Thermo Scientific™ SMART Digest™ Kits. A significant advance in sample preparation for biopharmaceutical and protein research, the kits provide fast and simple protein digestion with high reproducibility, high sensitivity, and high levels of data quality in a format that's compatible with automation. The SMART Digest Kits are a significant improvement over current in-solution protein digestion technologies, which are not reproducible, have poor sensitivity, and employ protracted methodologies not amenable to automation. Features: Easy and simple Significantly increased speed of sample processing. 3-step sample preparation. Easily automate entire protocol for high-throughput processing. Reliable results Increased reproducibility over existing protocols. Higher sensitivity. Higher data confidence.