Application Note: Selecting a Reversed-Phase Column for the Peptide Mapping Analysis of a Biotherapeutic Protein
27 November 2017

A chromatographic peptide mapping method must provide adequate resolution and recovery of a broad set of peptides if it is to be used in the quality analysis of a biotherapeutic protein. In this application note, a range of reversed phase columns by Waters are analysed for their applicability to peptide mapping, demonstrating high peak capacity separations.