Rapid characterization of oligonucleotides using microfluidic capillary electrophoresis

Oligonucleotides (oligos) represent a distinct class of therapeutics that include RNA, DNA, and their structural analogs, and are effective against a wide range of disease conditions. In this application note, 908 Devices presents a CE/ MS analysis of oligos by ZipChip coupled to a QExactive HF mass spectrometer. This positive ESI mode method utilizes an MS-friendly background electrolyte (BGE), that eliminates the use of ion-pairing agents such as hexafluoro isopropanol or alkyl amines.