Application Note: Optimize your IHC and IF for experimental success
2 June 2020
Immunohistochemistry (IHC) is a powerful technique used to detect and visualize proteins of interest within a cell or tissue using the specificity of the antigen-antibody reaction in combination with reporter molecules. Flow cytometry, enzyme-linked immunosorbent assays (ELISA) and western blotting are other widely used assay methods that are based upon the principle of antibody-antigen binding.
This application eBook presents a practical guide on how to increase specificity and enhance your labeling technique to achieve optimum lab success, including expert insight and guidance on:
- Control and blocking reagents
- Effective solutions to tackle species on species labeling
- The importance of understanding cross-reactivity and cross-adsorbed secondary antibodies