Increased Efficiency of Biomolecule Identification by Optimization of Trypsin Digestion Buffers
Increased Efficiency of Biomolecule Identification by Optimization of Trypsin Digestion Buffers
28 Oct 2015Protein insolubility can be a major impediment to traditional bottom-up proteomic methods because it reduces the efficiency of enzymatic digestions and makes analysis problematic. Insolubility can be caused by a variety of factors, including the salt concentration, the presence of various organic solvents, temperature, or simply the nature of the protein itself. This application note demonstrates how the Thermo Scientific™ SMART Digest™ kit removes the uncertainty associated with conventional solution-based tryptic digestion protocols, resulting in higher efficiency and sample characterization.
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Thermo Scientific™ SMART Digest™ Kits
Thermo Fisher ScientificObtain high quality analytical results from protein digests by using Thermo Scientific™ SMART Digest™ Kits. A significant advance in sample preparation for biopharmaceutical and protein research, the kits provide fast and simple protein digestion with high reproducibility, high sensitivity, and high levels of data quality in a format that's compatible with automation. The SMART Digest Kits are a significant improvement over current in-solution protein digestion technologies, which are not reproducible, have poor sensitivity, and employ protracted methodologies not amenable to automation. Features: Easy and simple Significantly increased speed of sample processing. 3-step sample preparation. Easily automate entire protocol for high-throughput processing. Reliable results Increased reproducibility over existing protocols. Higher sensitivity. Higher data confidence.