Application Note: Improving Preparative HPLC Column Lifetime in High-Throughput Open-Access Purification Environments
17 March 2017

When subjected to viscous samples under high-speed chromatographic conditions, the media bed of the column may deteriorate. This application note describes the use of Axia column technology to eliminate column voiding or chanelling, in conjunction with the SecurityGuard PREP system that protects the column from contaminants and particulates. This work demonstrates the dramatic increase in column lifetime achievable with these systems in place, even when operating under high flow rate, high-pressure conditions.