ResourceLife Sciences
IMAP Phosphodiesterase Assays on SpectraMax Multi-Mode Microplate Readers
3 Sept 2014This application note demonstrates how PDE enzyme dilution and inhibition curves are performed with IMAP Technology using the SpectraMax® i3, SpectraMax® Paradigm®, SpectraMax® M5, and FlexStation® 3 Multi-Mode Microplate Readers with SoftMax® Pro Software.
Links
Tags
Assay AssemblyAssay Assembly is technique used in drug discovery to develop assays to test the cytotoxicity, genotoxicity, or other activities of a compound on a cell. Assay assembly requires chip assembly, a delivery system and a detection and analysis method. Beneficial features of assay kits or automated systems include high-throughput, high speed and sensitivity and low signal to noise ratio.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Kinase AssaysAssay DevelopmentThe process of proving an assay to be sensitive with respect to the target is known as assay development. The assay should be able to characterize novel compounds and measure the potency of these compounds against a validated biological target.