Elispot Assays: State-of-the-Art Tools for Functional Analysis of Cellular Immunology

10 Jan 2013

Under optimal conditions, the enzyme-linked immunosorbent spot (Elispot) assay enables visualization of multiple secretory products from a single responding cell. Thus, the Elispot provides both qualitative and quantitative information. In this application note Millipore review and highlight the unique performance characteristics, workflow attributes and cost benefits which identify the Elispot assay as an excellent platform for elucidating the complexities underlying the immune responses.

Immobilon Transfer Membranes, Sandwiches, and Blotting Filter Paper

Merck

EMD Millipore offers three different Immobilon PVDF membranes, each optimized for different protein blotting applications. We also now offer blotting sandwiches that feature pre-cut sheets of membrane and blotting filter paper.Immobilon membranes provide a number of advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times

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Millex Filter Units

Merck

Millex filter units with hydrophobic Fluoropore membrane are ideal for sterilizing gases, venting sterile containers, and sterilizing or clarifying organic solutions. Specialized filter units protect hemodialysis transducers from blood and moisture. 50 mm Millex filter units are also used for vacuum line protection. All units are bi-directional

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Elispot Assays: State-of-the-Art Tools for Functional Analysis of Cellular Immunology