DNA Barcoding from Fish Tissues

Rapid and efficient extraction of DNA from a broad range of source material. KAPA Express Extract is a novel thermostable protease and buffer system that allows for the extraction of PCR-ready DNA from various tissue types in as little as 15 minutes. The KAPA Express Extract system has been designed for optimal tissue lysis and DNA preservation. DNA extractions are conveniently performed in a single-tube, without the need for hazardous chemicals and multiple washing steps, thereby greatly reducing the risk of sample loss and contamination. Key features of the KAPA Express Extract system include: Rapid extraction protocol - PCR-ready DNA in 15 minutes Versatility - single kit optimized for a variety of sample types Single-tube reaction minimizes risk of contamination Couple with KAPA2G Robust HotStart ReadyMix for increased PCR success rates The combination of KAPA Express Extract and KAPA2G Robust HotStart ReadyMix provides the highest performance solution for rapid DNA extraction and consistent downstream amplification. KAPA2G Robust DNA Polymerase is a novel enzyme evolved for increased processivity and exhibits greatly improved tolerance to carryover PCR inhibitors from crude DNA extractions.

Streamline your PCR workflows by consolidating protocols and reaction conditions, increase pass rates, amplify difficult templates without bias, and increase sensitivity – all with a single enzyme. The second-generation KAPA2G Robust DNA Polymerase was evolved to solve - achieve consistent amplification across a broad range of amplicon types (both GC and AT-rich). The versatility and robustness of the polymerase allows for consolidation of PCR cycling protocols and reaction conditions while increasing success rates. The high performance of the KAPA2G Robust DNA Polymerase eliminates the need for multiple enzymes and protocols - standardize your PCRs with a single enzyme solution. The KAPA2G Robust DNA Polymerase offers: Robust performance across a wide range of GC- and AT-rich templates Increased PCR success rates Consolidated PCR protocols and reaction conditions with a single enzyme Improved tolerance to common PCR inhibitors for crude samples and/or DNA extractions Consistent Colony PCR from E. coli and yeast Higher yield and sensitivity per unit of enzyme Three novel buffers and a proprietary PCR enhancer offer extended optimization options for the most difficult templates KAPA2G Robust DNA Polymerase is also available with HotStart technology for improved specificity during prolonged benchtop setup or liquid handling.