Application Note: Detection of HMT PRMT1 with the Transcreener® EPIGEN Methyltransferase Assay
25 August 2015

The Transcreener® EPIGEN Methyltransferase Assay is a universal biochemical HTS assay for enzymes that produce S-adenosylhomocysteine (SAH), including all enzymes within the histone (HMTs) and DNA (DNMTs) methyltransferase families. It combines the extensively validated Transcreener AMP2 which relies on fluorescent immunodetection of AMP, with coupling enzymes that convert SAH to AMP. This application note demonstrates a streamlined approach to develop a Transcreener EPIGEN Methyl transferase Assay for PRMT1. These steps can be followed to easily adapt the Transcreener EPIGEN Assay for doing high throughput screening with PRMT1.