Comparison of Assay Methods for the Detection of Residual Protein A in Biological Therapeutics
6 Apr 2015Protein A has been shown to leach from columns during purification of antibodies resulting in contamination. This poster provides a comparison of several suitable detection methods for the presence of residual Protein A in therapeutic biological agents including a homogenous proximity assay and conventional ELISA assay. The comparison includes evaluation of the lower detection limit of Protein A as well as screening results for detection of residual Protein A in a panel of nine human IgG antibodies.
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