Better Metagenomics Methods Offer Hope for Studies with Ultra-Low DNA
15 Sept 2013For low-input DNA studies such as viral metagenomics, there is no room for error in library protocols. This application note describes a case study in which scientists at the University of Arizona have optimized methods for sequencing sample prep. This study emphasizes the need for efficient and precise size selection.
Related Products
Request Quote for All Products
Pippin Prep DNA Size Selection System
Sage ScienceThe Pippin Prep is a preparative electrophoresis platform for collection of size-selected DNA samples. The system automates DNA size selection using disposable, pre-cast agarose cassettes to extract fractions according to user-defined software input. Samples are electro-eluted from agarose and collected in buffer. The platform is used for the construction of next-gen sequencing libraries. The system collects fragments (depending on gel type) between 90 bp- 8kb. A higher quality of sample can be extracted, more reproducibly and with no cross-contamination, when compared to manual gel purification methods.