Automated Extraction of High-Quality DNA from Tissue Samples Using sbeadex and oKtopure

The oKtopure™ is a fully automated nucleic acid extraction instrument that enables a new level of high-throughput processing and significant cost savings. This platform combines standardised high-throughput DNA or RNA extraction from a variety of sample types with the proprietary sbeadex™ magentic bead based extraction chemistry. The oKtopure is part of our on-going programme to provide customers with high productivity solutions, first developed and optimised for use within our service labs, where every day we deliver Extraction, Sequencing and Genotyping services. Key to the delivery of consistently high quality and high yield DNA preparations from the oKtopure is its ability to bind and hold the magnetic beads, and hence the DNA in place whilst transferring the buffers required for washing, binding and elution steps. The final aqueous wash used in the sbeadex extraction protocols enables the delivery of nucleic preparations ideal for downstream applications such as SNP genotyping, NGS and Sanger sequencing. Features: High throughput - capable of processing eight 96-well plates (768 samples) in approximately one-and-a-half hours Robust tip washing system reduces consumable costs by 50% or more Preloaded methods for different sample types – fully customizable Extraction expertise and support from scientists who use the instrument daily and have been carrying out high throughput extractions since 1996!

Our proprietary sbeadex magnetic microparticles are designed to deliver high yields of high quality DNA, and have long enabled high throughput automated processing.   sbeadex™ magnetic microparticles offer two key features which enable the extraction and purification of high quality DNA preparations: A novel two step binding mechanism via a patented adapter enables binding of nucleic acids more specifically. Final washes with pure water to deliver nucleic acids in high yield, purity and quality.   Features: Development of customised protocols to your requirements Tailor-made extractions like modified lysis or eluation steps according to user requirements Extraction speed optimisation - quicker results Highly flexible batch sizes   Technical advantages: Final washes with pure water to deliver nucleic acids in high yield, purity and quality No potential PCR inhibitors in wash buffers - e.g. organic solvents, salts, polyethyleneglycol Applicable for most popular robotic platforms (f.e. Hamilton, TECAN or KingFisher)   Available sbeadex kits: sbeadex plant kit sbeadex blood kit sbeadex plasmid kit sbeadex forensic kit sbeadex tissue kit sbeadex livestock kit sbeadex pathogen kit   The sbeadex technologies are suitable for high throughput automation and we have substantial experience developing sbeadex kits and protocols for use with a wide range of automation systems.