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Application Note: Virology & vaccine handbook
15 March 2023

Current methods to measure virus and neutralizing antibody titers include plaque assays, plaque reduction neutralization tests (PRNT), or immuno-focus assays. These methods are labor intensive and have inconsistent results due to the variable rate of plaque formation and optimal time points to count plaques for each infection.

In this handbook, Agilent describes how you can use the xCELLigence Real Time Cell Analysis instruments to track viral cytopathic effects continuously without the use of agar, dye, or fixative over minutes to days. These automated assays greatly reduce workload and provide quantitative kinetics for the entire virus life cycle.