Validation of the SureTect E. coli O157:H7 PCR Assay

24 Sept 2020

This study demonstrates the SureTect E. coli O157:H7 PCR method using the QuantStudio 5 PCR Instrument for PCR and RapidFinder Analysis software for data analysis is a suitable substitute to the reference methods for E. coli O157:H7 detection in raw ground beef, raw beef trim, spinach, and apple juice.

SimpliAmp™ Thermal Cycler

Applied Biosystems (Europe)

The SimpliAmp™ Thermal Cycler is a small, easy-to-use, and accurate thermal cycler that suits any lab's needs for everyday PCR. It is crafted with the reliable engineering and performance you’ve come to expect from Applied Biosystem® instruments. It is elegantly designed to deliver quiet cycling, and features an innovative, safe heated lid and a large LCD color touch screen. Key Features and Benefits: VeriFlex™ blocks to optimize PCR temperature with precision—the VeriFlex™ blocks provide 3 independent temperature zones, for precise temperature control over your PCR optimization. 8-inch color touch screen to help save time—the large and responsive color touch screen simplifies instrument operations with an easy to read interface. WiFi-enabled for remote monitoring—conveniently monitor your instrument from anywhere with our free PCR Essentials mobile app. Simple lid design to protect your sample—the innovative heated lid is easy to use and designed to help protect your samples. When open, it is in an upright position for easy handle access. Pull the handle forward to close the lid with ease. The heated lid will open automatically if not closed properly, to help safeguard your precious sample. Ready to use USB drive to easily back up your data—simply plug in a USB drive and follow the on-screen prompts to back up your data simply and effortlessly. Thermal simulation modes to replicate other PCR experiments—a library of simulation modes is available so that you may run an experiment using the same ramp rates as with a previous or different instrument.

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Validation of the SureTect E. coli O157:H7 PCR Assay