Total fat determination: A comparison study of conventional methods and microwave-assisted extraction with ETHOS X

1 Sept 2026

This application note compares conventional total fat determination methods with the microwave-assisted hydrolysis and extraction workflow enabled by the ETHOS™ X platform. Learn how the system delivers accurate and reproducible total fat measurements across a variety of food matrices, including dairy, meat, confectionery, spices and ready-to-eat products. Discover how simultaneous hydrolysis and extraction can reduce analysis time, simplify workflows, lower solvent consumption, and increase laboratory throughput while producing results comparable to established reference methods.

Resource details:

Resource type: Application note
Page count: 5
Read time: 7 mins

ETHOS X microwave extraction system for fat determination

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The process of fat determination in food and feed matrices is a routine analysis still relies on outdated and matrix-dependent methods which use obsolete and time-consuming technologies. The ETHOS X offers a new approach to gravimetric fat determination, moving toward a more modern, greener and more efficient approach to total fat, Fatty Acid Methyl Ester (FAME) and free fat determinations in food and feed matrices.

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Digestion and ExtractionDigestion and extraction apparatus are used in sample preparation to break down samples or extract organic or aqueous compounds from a solid. Digestion may be conducted via hot block, microwave or Kjeldahl digestion apparatus. Extraction systems typically involve solvent extraction and include Soxhlet, Randall or Twisselmann extraction methods. Find the best digestion and extraction apparatus in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.Microwave Sample Preparation