Application Note: Three’s Company: Formulation Prep and Stability Screening with Grunt, Lunatic and Uncle
26 December 2017
The stability of biologics can be highly dependent on formulation pH. The pH can be screened by buffer exchanging proteins into a variety of buffer salts and running follow-up analytics to determine stability. Current practices for buffer exchange all have limitations. Dialysis requires a large excess of buffer and time. Centrifugal filters and desalting columns are faster, but require more hands-on time. Additionally, all three methods require dilution or concentration steps to reach target protein concentrations. Automating buffer exchange eliminates these bottlenecks, making it easy to explore a greater experimental space.