Application Note: Perform a six-color probe-based assay without signal crosstalk and calibration effort
17 November 2023
Currently, most commercial detection assays identify up to four different targets, and rarely up to five genes. A major challenge lies in the precise separation of individual fluorescence signals across different channels. This is one of the main reasons why there are still relatively few assays using more than five different dyes for detection.
Thanks to the new technology featuring a unique multicolor LED light source and extended wavelength ranges in qTOWERiris, along with a clever combination of dyes used for probe labeling, real-time PCR with up to six targets, without crosstalk and the need for expensive calibration reagents, is now possible.