Multiple methods for normalizing cellular fluorescent signals to the cell number per well

21 Apr 2022

Quantitative cell-based experiments are crucial for the analysis of cellular response to stimulation. This technical note describes how to perform quantitative cell-based analyses using a multimode plate reader with whole-well imaging capabilities.

Spark® Cyto

Tecan

Spark Cyto is the first live cell plate reader to detect biological, chemical or physical events – capturing the maximum amount of data possible from each plate well, at the same time and under the same conditions.

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Multiple methods for normalizing cellular fluorescent signals to the cell number per well