Multiple focused CRISPR libraries from a single oligo pool
12 May 2021Functional genetic screening using the CRISPR/Cas9 system is a powerful tool for interrogating gene function at scale, but access to and implementation of this technology is constrained by existing pre-defined CRISPR sgRNA libraries.
In this application note, a single Twist Oligo Pool was used in combination with Custom Library Multiplexed Cloning, an easy-to-use bioinformatics and wet-lab workflow, to generate numerous high-quality, custom sgRNA libraries.
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Twist BioscienceTwist Oligo Pools are highly diverse collections of single-stranded oligonucleotides synthesized using our silicon-based DNA writing technology. Our synthesis platform enables massively parallel production of hundreds of thousands of high-quality, accurate oligos per run.
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