Application Note: Meet Big Tuna: Automated, Versatile Buffer Exchange
16 November 2018

Buffer exchange with Big Tuna is highly customizable and adaptable, allowing for buffer exchange of up to 96 unique proteins and formulations in a single experiment. Unchained Labs have developed two filter plate formats for this process. The Unfilter 96 and 24 are 10 kDa molecular weight cut-off (MWCO) filtration plates which are designed to withstand 60 psi pressurization during the buffer exchange process. Unfilter 96 can process up to 96 samples ranging from 100-450 µL; Unfilter 24 can process 24 samples ranging from 0.45-8 mL in a single run.