Application Note: In Situ Raman Study on Polysorbate Induced Protein Agglomeration with Hound
21 September 2018

Polysorbate is a surfactant commonly used to stabilize protein formulations. Degradation of polysorbates via hydrolysis causes the formation of fatty acids. This degradation is a potential trigger for protein aggregation since insoluble fatty acid particles can induce agglomeration. This application note describes how you can use Hound to count, size and identify protein particles resulting from surfactant degradation.