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Comparison of handling, time-to-result and validation parameters of commercially available PCR methods for Salmonella species detection and identification

Thermo Fisher Scientific
8 Sept 2020

This application note compares the Thermo Scientific™ RapidFinder™ Salmonella species, Typhimurium and Enteritidis Multiplex PCR Kit to four other commercially available PCR methods. PCR kits are compared for differences in necessary equipment, bench-space needed, number and duration for sample handling steps, method complexity and certification provided by AOAC-RI and AFNOR NF VALIDATION. The PCR methods are also compared for multiplex capability and total sample throughput.

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Thermo Scientific™ RapidFinder™ Salmonella Species, Typhimurium and Enteritidis Multiplex PCR Kits

Thermo Fisher Scientific

Rapidly detect and differentiate Salmonella species, Salmonella ser. Typhimurium, and Salmonella ser. Enteritidis in a single test with Thermo Scientific™ RapidFinder™ Salmonella Species, Typhimurium and Enteritidis Multiplex PCR Kit. Confidently test accuracy and speed up product release with results in as little as 16 to 22 hours and validated workflows for poultry and pork meats, production environment samples and primary production samples. The kits are compatible with the Appli

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MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Food and Beverage ProcessingFood and Beverage Processing involves the preparation of food products for further analysis. Typical instrumentation includes blanchers, color sorters, cookers, grinders, and refrigeration. These are used to replicate the processes commonly used in the commercial preparation of food and beverages.Food SafetyFood safety describes the prevention of food-borne illnesses. Contamination with a variety of pathogens can be prevented with adequate temperature control, hygiene and labeling. Food samples can be tested using GC/MS, HPLC, SPE, and qPCR to ensure their adherence to regulations.Food TestingFood testing refers to a variety experiments including PCR, mass spectrometry, processing, QuEChERS, analyzing and Kjeldahl.Food ContaminationFood contamination refers to the presence of harmful substances or pathogens in food, posing risks to human health. Contaminants can be biological, chemical, or physical, and their detection is crucial for ensuring food safety. Advanced testing methods like PCR, chromatography, and spectroscopy are used to identify contaminants in food products. Browse our peer-reviewed product directory to find the best solutions for food contamination detection, compare products, check reviews, and get pricing directly from manufacturers.

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Comparison of handling, time-to-result and validation parameters of commercially available PCR methods for Salmonella species detection and identification