Application Note: Cardiomyocyte Beating and Cytotoxicity Assays on the SpectraMax i3 Multi-Mode Microplate Platform with SpectraMax MiniMax Imaging Cytometer
10 April 2019
Stem cell-derived human cardiomyocytes have phenotypic characteristics and electrophysiological profiles similar to those of native human cardiac cells. Cardiomyocytes in culture are able to form a beating syncytium, which behaves similarly to native cardiomyocytes. Oscillation of intracellular calcium levels occurring with synchronized contractions of the cells can be monitored using a calciumsensitive dye, and treatment-induced changes in the pattern of oscillation can be monitored via changes in fluorescent signal over time.