Application Note: Brightfield and fluorescence imaging using 3D PrimeSurface ultra-low attachment microplates
24 August 2019

3D cell culture has become a well-established in vitro experimental approach as it realistically mimics an in vivo environment. The use of clear U-bottom ultra-low attachment microplates, that minimize cell adherence, has become a standard for applications such as spheroid proliferation. In this application note, BioTek presents data generated with the Cytation™ 5 Cell Imaging Multi-Mode Reader, using PrimeSurface ULA plates to develop simple and robust spheroid assays for brightfield and fluorescence imaging.