Automating library prep for NGS-based genetic testing

20 Feb 2020

Many common bottlenecks and quality problems in the next-generation sequencing (NGS) workflow can be traced to the laborious and time-consuming task of preparing sequencing-ready libraries. In this guide, Tecan explains how automating library prep can help improve confidence in the NGS pipeline, and what to consider when choosing automation solutions.

Fluent® Laboratory Automation Workstation for Genomic Workflows

Tecan

Fluent’s groundbreaking design delivers more capacity and productivity for genomic workflows. This innovative workstation offers superior precision, higher throughput and longer walkaway times, allowing scientists to get more done in less time, with greater confidence. The Fluent Laboratory Automation Workstation for genomic workflows offers high precision liquid handling – down to 200 nl – for accurate quantification set-up and normalization of DNA, as well as PCR/qPCR set-up and NGS library preparation. It’s freely configurable architecture allows integration of devices for molecular biology protocols, and can easily be adapted to additional workflows. Whether you want to increase throughput or reduce variability for nucleic acid purification, quantification and normalization or reaction set-up, Fluent provides greater throughput and more consistent results.   The platform’s intuitive FluentControl™ software allows protocols to be written for virtually any genomics application, with dedicated commands for creating standard curves, DNA/RNA normalization, etc. An Active Stop and Resume function permits instant access to the workdeck during a run – allowing set-up errors or omissions to be corrected simply by opening the door – while advanced Method Recovery options further enhance process security. Features: Choice between liquid and air displacement technologies Minimizes risk of errors with smaller sample volumes Built in touchscreen interface Enhanced process security FluentControl Software Integration options  

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Clinical GeneticsMolecular Genetics covers the analysis of hereditary genetic disease and chromosomal abnormalities. Genetics can be analysed using DNA, RNA, and protein microarrays, PCR, RT PCR and DNA sequencing. Genetic equipment includes genetic workstations, thermal cyclers, cooling blocks and electrophoresis products. Diagnostic kits are used for DNA / RNA extraction and purification.Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.AutomationAutomation in laboratories and manufacturing processes enhances efficiency, precision, and scalability by reducing the need for manual intervention. It plays a critical role in improving productivity, minimizing human error, and accelerating workflows in fields like diagnostics, drug development, and industrial testing. Automation technologies include robotic systems, automated liquid handlers, and process control systems that streamline complex tasks and ensure consistent, reproducible results. Explore our peer-reviewed product directory to discover the best automation solutions, compare options, read user reviews, and get prices directly from manufacturers.Clinical NGSNext Generation Sequencing (NGS) refers to the use of massive parallel sequencing of multiple small fragments of DNA. This high-throughput genomic analysis yields enormous amounts of sequence data, which if appropriately analyzed could have huge potential for clinical laboratories. For this to happen there are technique and bioinformatic hurdles to be overcome.Clinical AutomationClinical laboratory automation refers to the use of automated systems in clinical laboratories to increase efficiency, reduce human error, and improve patient care. From sample analysis to total laboratory automation, this plays a critical role in modern diagnostics and laboratory workflows. Explore clinical automation solutions in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.NGS AutomationNGS automation streamlines next-generation sequencing workflows, increasing throughput, accuracy, and reproducibility. Automated solutions enhance productivity in genomic research, clinical diagnostics, and personalized medicine. Browse peer-reviewed tools to compare products, read reviews, and get pricing.Genetics Automation